Show simple item record

hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorDe Mariano, Marilena
hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorStigliani, Sara
hal.structure.identifierLaboratoire d'analyse et modélisation de systèmes pour l'aide à la décision [LAMSADE]
dc.contributor.authorMoretti, Stefano
HAL ID: 739814
ORCID: 0000-0003-3627-3257
hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorParodi, Federica
hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorCroce, Michela
hal.structure.identifier
dc.contributor.authorBernardi, Cinzia
hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorPagano, Aldo
hal.structure.identifierPediatric Research Institute
dc.contributor.authorTonini, Gian Paolo
hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorFerrini, Silvano
hal.structure.identifierIstituto Nazionale per la Ricerca sul Cancro, Genoa
dc.contributor.authorLongo, Luca
dc.date.accessioned2017-11-07T16:24:31Z
dc.date.available2017-11-07T16:24:31Z
dc.date.issued2017
dc.identifier.issn1949-2553
dc.identifier.urihttps://basepub.dauphine.fr/handle/123456789/16928
dc.language.isoenen
dc.subjectneuroblastomaen
dc.subjectmicroRNAen
dc.subjectALKen
dc.subjectmiR-424-5pen
dc.subjectmiR-503-5pen
dc.subject.ddc515en
dc.titleA genome-wide microRNA profiling indicates miR-424-5p and miR-503-5p as regulators of ALK expression in neuroblastomaen
dc.typeArticle accepté pour publication ou publié
dc.description.abstractenThe discovery of missense mutations of ALK gene identified this receptor tyrosine kinase as a therapeutic target in neuroblastoma (NB). Moreover, a high level of ALK protein has been associated with metastatic NB cases and with a worse prognosis, suggesting that also ALK overexpression is involved in NB tumorigenesis. Since miRNAs play key roles in the regulation of gene expression we aimed at identifying those miRNAs that can regulate ALK in NB. We therefore analyzed the genome-wide expression profile of miRNAs in two sample sets of 16 NB cell lines and 22 NB samples by using miRNA microarrays. Both sample sets were then divided into two subgroups showing high (ALK+) or low/absent (ALK-) expression of ALK. Results showed a down-regulation of 30 and 23 miRNAs (p-value <0.05) in the ALK+ group in NB cell lines and samples, respectively. Validation analysis indicated that miR-424-5p and miR-503-5p, belonging to the same cluster, were differentially expressed in both NB cell lines and tumor samples. Although only miR-424-5p showed a direct binding to ALK 3′-UTR, both miRNAs led to a remarkable decreasing of ALK protein as well as to the inhibition of cell viability in ALK+ NB cell lines. In conclusion, our data indicate that both miR-424-5p and miR-503-5p are involved in regulating ALK expression in NB, either by directly targeting ALK receptor or indirectly, and may thus serve as potential therapeutic tools in ALK dependent NBs.en
dc.relation.isversionofjnlnameOncotarget
dc.relation.isversionofjnlvol8en
dc.relation.isversionofjnlissue34en
dc.relation.isversionofjnldate2017
dc.relation.isversionofjnlpages56518–56532en
dc.relation.isversionofdoi10.18632/oncotarget.17033en
dc.subject.ddclabelAnalyseen
dc.relation.forthcomingnonen
dc.relation.forthcomingprintnonen
dc.description.ssrncandidatenonen
dc.description.halcandidateouien
dc.description.readershiprechercheen
dc.description.audienceInternationalen
dc.relation.Isversionofjnlpeerreviewedouien
dc.relation.Isversionofjnlpeerreviewedouien
dc.date.updated2017-11-07T16:09:05Z
hal.identifierhal-01630524*
hal.version1*
hal.update.actionupdateFiles*
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut
hal.author.functionaut


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record